Journal: iScience

Article Title: Colitis-induced upregulation of tumor necrosis factor receptor-2 (TNFR2) terminates epithelial regenerative signaling to restore homeostasis

doi: 10.1016/j.isci.2023.107829

Figure Lengend Snippet:

Article Snippet: Alexa Fluor 488 Rat anti-Mouse Ly-6A/E (Sca1) , Southern Biotechnology Associates , RRID: AB_175530.

Techniques: Recombinant, Plasmid Preparation, RNAscope, Software

Journal: Nature Communications

Article Title: Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses

doi: 10.1038/s41467-019-11002-5

Figure Lengend Snippet: Iron deficiency results in impaired TD and TI immune responses in mice. Three-week-old wild-type C57BL/6J mice (male) were divided into two groups and fed either a control iron-adequate diet (45.4 mg/kg iron) or an iron-deficient diet (0.9 mg/kg iron) for 5 weeks. a Flow cytometry of splenocytes from control and iron-deficient mice stained with anti-B220 to identify peripheral B cells. Bone marrow cells from control and iron-deficient mice were stained with anti-B220, anti-IgM, and anti-IgD antibodies to assess pre- and pro-B cells (IgM − IgD − ), immature B cells (IgM + IgD int ), and mature circulating B cells (IgM + IgD hi ). b Statistics for the numbers of splenic B cells in the two groups (mean ± SEM of three mice per group). c – e Antigen-specific antibody responses of control and iron-deficient mice immunized with DNP-KLH (TD), DNP-Ficoll (TI-2), or TNP-LPS (TI-1). Serum was collected preimmunization and at 7 days and 21 days after immunization. In the case of DNP-KLH, the mice were rechallenged at day 21 as indicated by the arrow, and serum was collected at day 28 and to a long-term at day 60 (the data represent the mean ± SEM of four mice per group). f Control and iron-deficient mice were immunized with SRBCs, DNP-KLH or PBS as a control, and at day 10, splenocytes were stained with anti-B220, anti-IgD, anti-GL7, and anti-CD95 (Fas) antibodies to assess the GCB cells (B220 + IgD low GL7 + Fas + ). g Statistics for the proportions of GCB cells in mice immunized with DNP-KLH or SRBCs (mean ± SEM of three mice per group). h Frozen sections of spleens from mice immunized with SRBCs were stained with anti-IgD antibody and PNA to identify germinal center reactions. The data were representative of at least two independent experiments. * P < 0.05, ** P < 0.01, Student’s t -test

Article Snippet: The sections were then washed with PBS containing 0.5% (vol/vol) Tween and incubated with secondary antibodies for 2 h. The antibodies included Alexa Fluor 488-conjugated rat anti-IgD (BD Pharmingen), biotin-conjugated peanut agglutinin (PNA, Vector Laboratories), and Alexa Fluor 555-conjugated streptavidin (Invitrogen).

Techniques: Flow Cytometry, Staining